Immune activation mediated by the late blight resistance protein R1 requires nuclear localization of R1 and the effector AVR1.
Identifieur interne : 000E42 ( Main/Exploration ); précédent : 000E41; suivant : 000E43Immune activation mediated by the late blight resistance protein R1 requires nuclear localization of R1 and the effector AVR1.
Auteurs : Yu Du [Pays-Bas] ; Jeroen Berg [Pays-Bas] ; Francine Govers [Pays-Bas] ; Klaas Bouwmeester [Pays-Bas]Source :
- The New phytologist [ 1469-8137 ] ; 2015.
Descripteurs français
- KwdFr :
- Facteurs de virulence (métabolisme), Fluorescence (MeSH), Maladies des plantes (immunologie), Maladies des plantes (microbiologie), Mort cellulaire (MeSH), Noyau de la cellule (métabolisme), Phytophthora infestans (métabolisme), Protéines à fluorescence verte (métabolisme), Résistance à la maladie (MeSH), Signaux d'export nucléaire (MeSH), Tabac (immunologie), Tabac (microbiologie), Transport des protéines (MeSH).
- MESH :
- immunologie : Maladies des plantes, Tabac.
- microbiologie : Maladies des plantes, Tabac.
- métabolisme : Facteurs de virulence, Noyau de la cellule, Phytophthora infestans, Protéines à fluorescence verte.
- Fluorescence, Mort cellulaire, Résistance à la maladie, Signaux d'export nucléaire, Transport des protéines.
English descriptors
- KwdEn :
- Cell Death (MeSH), Cell Nucleus (metabolism), Disease Resistance (MeSH), Fluorescence (MeSH), Green Fluorescent Proteins (metabolism), Nuclear Export Signals (MeSH), Phytophthora infestans (metabolism), Plant Diseases (immunology), Plant Diseases (microbiology), Protein Transport (MeSH), Tobacco (immunology), Tobacco (microbiology), Virulence Factors (metabolism).
- MESH :
- chemical , metabolism : Green Fluorescent Proteins, Virulence Factors.
- immunology : Plant Diseases, Tobacco.
- metabolism : Cell Nucleus, Phytophthora infestans.
- microbiology : Plant Diseases, Tobacco.
- Cell Death, Disease Resistance, Fluorescence, Nuclear Export Signals, Protein Transport.
Abstract
Resistance against oomycete pathogens is mainly governed by intracellular nucleotide-binding leucine-rich repeat (NLR) receptors that recognize matching avirulence (AVR) proteins from the pathogen, RXLR effectors that are delivered inside host cells. Detailed molecular understanding of how and where NLR proteins and RXLR effectors interact is essential to inform the deployment of durable resistance (R) genes. Fluorescent tags, nuclear localization signals (NLSs) and nuclear export signals (NESs) were exploited to determine the subcellular localization of the potato late blight protein R1 and the Phytophthora infestans RXLR effector AVR1, and to target these proteins to the nucleus or cytoplasm. Microscopic imaging revealed that both R1 and AVR1 occurred in the nucleus and cytoplasm, and were in close proximity. Transient expression of NLS- or NES-tagged R1 and AVR1 in Nicotiana benthamiana showed that activation of the R1-mediated hypersensitive response and resistance required localization of the R1/AVR1 pair in the nucleus. However, AVR1-mediated suppression of cell death in the absence of R1 was dependent on localization of AVR1 in the cytoplasm. Balanced nucleocytoplasmic partitioning of AVR1 seems to be a prerequisite. Our results show that R1-mediated immunity is activated inside the nucleus with AVR1 in close proximity and suggest that nucleocytoplasmic transport of R1 and AVR1 is tightly regulated.
DOI: 10.1111/nph.13355
PubMed: 25760731
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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type="city">Wageningue</settlement><region nuts="2">Gueldre (province)</region></placeName></affiliation></author><author><name sortKey="Berg, Jeroen" sort="Berg, Jeroen" uniqKey="Berg J" first="Jeroen" last="Berg">Jeroen Berg</name><affiliation wicri:level="4"><nlm:affiliation>Laboratory of Phytopathology, Wageningen University, Wageningen, the Netherlands.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Laboratory of Phytopathology, Wageningen University, Wageningen</wicri:regionArea><orgName type="university">Université de Wageningue</orgName><placeName><settlement type="city">Wageningue</settlement><region nuts="2">Gueldre (province)</region></placeName></affiliation></author><author><name sortKey="Govers, Francine" sort="Govers, Francine" uniqKey="Govers F" first="Francine" last="Govers">Francine Govers</name><affiliation wicri:level="4"><nlm:affiliation>Laboratory of Phytopathology, Wageningen University, Wageningen, the Netherlands.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Laboratory of Phytopathology, Wageningen University, Wageningen</wicri:regionArea><orgName type="university">Université de Wageningue</orgName><placeName><settlement type="city">Wageningue</settlement><region nuts="2">Gueldre (province)</region></placeName></affiliation></author><author><name sortKey="Bouwmeester, Klaas" sort="Bouwmeester, Klaas" uniqKey="Bouwmeester K" first="Klaas" last="Bouwmeester">Klaas Bouwmeester</name><affiliation wicri:level="4"><nlm:affiliation>Laboratory of Phytopathology, Wageningen University, Wageningen, the Netherlands.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">Pays-Bas</country><wicri:regionArea>Laboratory of Phytopathology, Wageningen University, Wageningen</wicri:regionArea><orgName type="university">Université de Wageningue</orgName><placeName><settlement type="city">Wageningue</settlement><region nuts="2">Gueldre (province)</region></placeName></affiliation></author></analytic><series><title level="j">The New phytologist</title><idno type="eISSN">1469-8137</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell Death (MeSH)</term><term>Cell Nucleus (metabolism)</term><term>Disease Resistance (MeSH)</term><term>Fluorescence (MeSH)</term><term>Green Fluorescent Proteins (metabolism)</term><term>Nuclear Export Signals (MeSH)</term><term>Phytophthora infestans (metabolism)</term><term>Plant Diseases (immunology)</term><term>Plant Diseases (microbiology)</term><term>Protein Transport (MeSH)</term><term>Tobacco (immunology)</term><term>Tobacco (microbiology)</term><term>Virulence Factors (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Facteurs de virulence (métabolisme)</term><term>Fluorescence (MeSH)</term><term>Maladies des plantes (immunologie)</term><term>Maladies des plantes (microbiologie)</term><term>Mort cellulaire (MeSH)</term><term>Noyau de la cellule (métabolisme)</term><term>Phytophthora infestans (métabolisme)</term><term>Protéines à fluorescence verte (métabolisme)</term><term>Résistance à la maladie (MeSH)</term><term>Signaux d'export nucléaire (MeSH)</term><term>Tabac (immunologie)</term><term>Tabac (microbiologie)</term><term>Transport des protéines (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Green Fluorescent Proteins</term><term>Virulence Factors</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Maladies des plantes</term><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Plant Diseases</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Nucleus</term><term>Phytophthora infestans</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Maladies des plantes</term><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Facteurs de virulence</term><term>Noyau de la cellule</term><term>Phytophthora infestans</term><term>Protéines à fluorescence verte</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cell Death</term><term>Disease Resistance</term><term>Fluorescence</term><term>Nuclear Export Signals</term><term>Protein Transport</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Fluorescence</term><term>Mort cellulaire</term><term>Résistance à la maladie</term><term>Signaux d'export nucléaire</term><term>Transport des protéines</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Resistance against oomycete pathogens is mainly governed by intracellular nucleotide-binding leucine-rich repeat (NLR) receptors that recognize matching avirulence (AVR) proteins from the pathogen, RXLR effectors that are delivered inside host cells. Detailed molecular understanding of how and where NLR proteins and RXLR effectors interact is essential to inform the deployment of durable resistance (R) genes. Fluorescent tags, nuclear localization signals (NLSs) and nuclear export signals (NESs) were exploited to determine the subcellular localization of the potato late blight protein R1 and the Phytophthora infestans RXLR effector AVR1, and to target these proteins to the nucleus or cytoplasm. Microscopic imaging revealed that both R1 and AVR1 occurred in the nucleus and cytoplasm, and were in close proximity. Transient expression of NLS- or NES-tagged R1 and AVR1 in Nicotiana benthamiana showed that activation of the R1-mediated hypersensitive response and resistance required localization of the R1/AVR1 pair in the nucleus. However, AVR1-mediated suppression of cell death in the absence of R1 was dependent on localization of AVR1 in the cytoplasm. Balanced nucleocytoplasmic partitioning of AVR1 seems to be a prerequisite. Our results show that R1-mediated immunity is activated inside the nucleus with AVR1 in close proximity and suggest that nucleocytoplasmic transport of R1 and AVR1 is tightly regulated. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25760731</PMID><DateCompleted><Year>2016</Year><Month>04</Month><Day>26</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1469-8137</ISSN><JournalIssue CitedMedium="Internet"><Volume>207</Volume><Issue>3</Issue><PubDate><Year>2015</Year><Month>Aug</Month></PubDate></JournalIssue><Title>The New phytologist</Title><ISOAbbreviation>New Phytol</ISOAbbreviation></Journal><ArticleTitle>Immune activation mediated by the late blight resistance protein R1 requires nuclear localization of R1 and the effector AVR1.</ArticleTitle><Pagination><MedlinePgn>735-47</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/nph.13355</ELocationID><Abstract><AbstractText>Resistance against oomycete pathogens is mainly governed by intracellular nucleotide-binding leucine-rich repeat (NLR) receptors that recognize matching avirulence (AVR) proteins from the pathogen, RXLR effectors that are delivered inside host cells. Detailed molecular understanding of how and where NLR proteins and RXLR effectors interact is essential to inform the deployment of durable resistance (R) genes. Fluorescent tags, nuclear localization signals (NLSs) and nuclear export signals (NESs) were exploited to determine the subcellular localization of the potato late blight protein R1 and the Phytophthora infestans RXLR effector AVR1, and to target these proteins to the nucleus or cytoplasm. Microscopic imaging revealed that both R1 and AVR1 occurred in the nucleus and cytoplasm, and were in close proximity. Transient expression of NLS- or NES-tagged R1 and AVR1 in Nicotiana benthamiana showed that activation of the R1-mediated hypersensitive response and resistance required localization of the R1/AVR1 pair in the nucleus. However, AVR1-mediated suppression of cell death in the absence of R1 was dependent on localization of AVR1 in the cytoplasm. Balanced nucleocytoplasmic partitioning of AVR1 seems to be a prerequisite. Our results show that R1-mediated immunity is activated inside the nucleus with AVR1 in close proximity and suggest that nucleocytoplasmic transport of R1 and AVR1 is tightly regulated. </AbstractText><CopyrightInformation>© 2015 The Authors New Phytologist © 2015 New Phytologist Trust.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Du</LastName><ForeName>Yu</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Laboratory of Phytopathology, Wageningen University, Wageningen, the Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Berg</LastName><ForeName>Jeroen</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Laboratory of Phytopathology, Wageningen University, Wageningen, the Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Govers</LastName><ForeName>Francine</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Laboratory of Phytopathology, Wageningen University, Wageningen, the Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bouwmeester</LastName><ForeName>Klaas</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Laboratory of Phytopathology, Wageningen University, Wageningen, the Netherlands.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>03</Month><Day>11</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>New Phytol</MedlineTA><NlmUniqueID>9882884</NlmUniqueID><ISSNLinking>0028-646X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D049790">Nuclear Export Signals</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D037521">Virulence Factors</NameOfSubstance></Chemical><Chemical><RegistryNumber>147336-22-9</RegistryNumber><NameOfSubstance UI="D049452">Green Fluorescent Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D016923" MajorTopicYN="N">Cell Death</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002467" MajorTopicYN="N">Cell Nucleus</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D060467" MajorTopicYN="Y">Disease Resistance</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005453" MajorTopicYN="N">Fluorescence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D049452" MajorTopicYN="N">Green Fluorescent Proteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D049790" MajorTopicYN="N">Nuclear Export Signals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055750" MajorTopicYN="N">Phytophthora infestans</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D021381" MajorTopicYN="N">Protein Transport</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D037521" MajorTopicYN="N">Virulence Factors</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Phytophthora infestans</Keyword><Keyword MajorTopicYN="N">RXLR effector</Keyword><Keyword MajorTopicYN="N">effector-triggered immunity (ETI)</Keyword><Keyword MajorTopicYN="N">nucleocytoplasmic partitioning</Keyword><Keyword MajorTopicYN="N">nucleotide-binding leucine-rich repeat (NLR)</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2014</Year><Month>12</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>02</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>3</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>3</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>4</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25760731</ArticleId><ArticleId IdType="doi">10.1111/nph.13355</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Pays-Bas</li></country><region><li>Gueldre (province)</li></region><settlement><li>Wageningue</li></settlement><orgName><li>Université de Wageningue</li></orgName></list><tree><country name="Pays-Bas"><region name="Gueldre (province)"><name sortKey="Du, Yu" sort="Du, Yu" uniqKey="Du Y" first="Yu" last="Du">Yu Du</name></region><name sortKey="Berg, Jeroen" sort="Berg, Jeroen" uniqKey="Berg J" first="Jeroen" last="Berg">Jeroen Berg</name><name sortKey="Bouwmeester, Klaas" sort="Bouwmeester, Klaas" uniqKey="Bouwmeester K" first="Klaas" last="Bouwmeester">Klaas Bouwmeester</name><name sortKey="Govers, Francine" sort="Govers, Francine" uniqKey="Govers F" first="Francine" last="Govers">Francine Govers</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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